bmdm cell line Search Results


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BEI Resources bmdm cell line
Effect of yolkin on viability and proliferation of bone <t>marrow-derived</t> <t>macrophages</t> <t>(BMDM).</t> The cells (1 × 10 5 /mL) were seeded in 96-well plates in DMEM + 10% FBS and incubated overnight at 37 °C. Next, the cells were exposed to yolkin (10, 100 and 150 µg/mL) for 24 and 48 h. The cell proliferation activity of yolkin was assessed with an MTT assay. Non-stimulated cells were used as a negative control. The results represent three to five independent experiments and data are presented as median ± min–max. A one-sample t -test was used to examine the mean differences between samples and control (100). * p ≤ 0.05, *** p ≤ 0.0001.
Bmdm Cell Line, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of yolkin on viability and proliferation of bone marrow-derived macrophages (BMDM). The cells (1 × 10 5 /mL) were seeded in 96-well plates in DMEM + 10% FBS and incubated overnight at 37 °C. Next, the cells were exposed to yolkin (10, 100 and 150 µg/mL) for 24 and 48 h. The cell proliferation activity of yolkin was assessed with an MTT assay. Non-stimulated cells were used as a negative control. The results represent three to five independent experiments and data are presented as median ± min–max. A one-sample t -test was used to examine the mean differences between samples and control (100). * p ≤ 0.05, *** p ≤ 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: A Novel Mechanism of Macrophage Activation by the Natural Yolkin Polypeptide Complex from Egg Yolk

doi: 10.3390/ijms23063125

Figure Lengend Snippet: Effect of yolkin on viability and proliferation of bone marrow-derived macrophages (BMDM). The cells (1 × 10 5 /mL) were seeded in 96-well plates in DMEM + 10% FBS and incubated overnight at 37 °C. Next, the cells were exposed to yolkin (10, 100 and 150 µg/mL) for 24 and 48 h. The cell proliferation activity of yolkin was assessed with an MTT assay. Non-stimulated cells were used as a negative control. The results represent three to five independent experiments and data are presented as median ± min–max. A one-sample t -test was used to examine the mean differences between samples and control (100). * p ≤ 0.05, *** p ≤ 0.0001.

Article Snippet: The murine bone marrow-derived macrophages of the BMDM cell line (Bei Resources) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS, antibiotics (penicillin, streptomycin and gentamycin) and 3% L-glutamine.

Techniques: Derivative Assay, Incubation, Activity Assay, MTT Assay, Negative Control, Control